摘要
利用毛地黄苷从菠菜叶绿体类囊体膜制备了PSⅡ颗粒,氧化还原差示光谱及SDS-聚丙烯酰胺凝胶电泳结果表明其具备PSⅡ的典型特征,它具有从水氧化到质醌还原的酶活性。从大豆磷脂用超声法制备了脂质体。从鼠肝线粒体分离了嵴膜。将制备的PSⅡ颗粒预组装于脂质体,然后将此预组装物(PSⅡ—PL)再与嵴膜组合,此膜系于光下获得了相当量的ATP合成,证明了融合膜中PSⅡ电子传递可推动嵴膜的电子传递和磷酸化机构合成ATP。
PS Ⅱ particles were prepared bydigitonin method fron spinach chloro-plasts (Plate Ⅰ-1). These particleshad the activity of all enzymes catlyz-ing the reaction from water oxidationto plastoquinone reduction (Fig. 2),and had PS Ⅱ activtiy as evidence bythe differential spectra of oxidation andreduction-A_(680) (Fig. 3), and the elec-trophoretogram on SDS-polyacry-lamide plate (Fig. 4). Liposomes wereprepared by sonication method fromthe phospholipids of soybean (PlateⅠ-2), and the crista membranes wereisolated from rat liver mitochondria.The PS Ⅱ particles were preassembledto the liposomes to form the PS Ⅱ-PLparticles (Plate Ⅰ-3), which wasthen combined with crista membranes(Plate Ⅰ-4). The reconstituted mem-brane system could synthesize ATP inlight in an adquate quantity (Table1). It has been proved that the PS Ⅱelectron flow from fusion membranescan drive the e1ectron transport ofcrista membranes and couple phospho-rylation with it in the reconstitutedmembrane system.
关键词
光合系统
磷酸化
嵴膜
电子传递
PS Ⅱ particles
crista membranes
assembling
electron transport
ATP synthesis
