摘要
用大赖草(Leymus tacemosus(Lam.)Tzvel.=L.giganteus)成熟胚愈伤组织经AA和DM培养基诱导出结构疏松的胚性愈伤组织,并以此建立了胚性细胞悬浮系。用悬浮系细胞游离原生质体,迸行浅层和双层培养。当形成见愈伤组织后转入固体培养基NBI或液体培养基MBL增殖,随后转到NBⅡ或NR培养基诱导分化。待形成绿色芽点后转到含NAA 0.5 mg/L的MSⅡ培养基上得到同时具有芽和根的再生植株。突验结果还表明,Km8p培养基对大赖草原生质体的早期培养具有良好效果。此外,加液培养基的成分对原生质体培养有影响,说明原生质体发育的不同时期所需的营养条件是有差异的。
Calli initiated from mature embryos of Leymus racemosus (Lam.)Tzvel. =L. giganteus were transferred onto the AA and DM media to produce friable embryogenic callus,from which em- bryogenic suspension cultures were established. Protoplasts were isolated from the embryogenic suspension cultures and were cultured either in thin-layer liquid medium or in double-layer (a- gar/liquid) medium. When visible calli were formed they were transferred onto the NBI agar medium or into the MBL liquid medium for further proliferation. These calli were transferred onto differentiation media of NBII and NR,where green spots were developed. Plants with both shoots and roots can be recovered from these green spots on MS Ⅱ medium containing 0.5 mg/L NAA. The results showed that the Km8p basal medium was favourable to the culture of L. racemosus protoplasts during the early stages of culture. In addition, the composition of the media added to the cultures had a marked influence on the growth of protoplasts, indicating that the nutritional requirements in this plant were different at various stages of protoplast growth and differentia- tion.
关键词
大赖草
原生质体培养
植株再生
Leymus racemosus
Protoplast
Plant regeneration
Cell suspension
