摘要
目的研制粪经蛋白质检测芯片及其初步应用。方法以抗原抗体反应为原理建立了粪便人血红蛋白、白蛋白、幽门螺杆菌全菌抗原、致病性大肠杆菌K-12、CEA、P53、Ras21、QYC抗原、蛔虫卵、钩虫卵、白细胞抗原等蛋白质检测芯片。结果该蛋白芯片各个蛋白抗原的检测下限灵敏度均不同,血红蛋白为50ng/ml,白蛋白为1000ng/ml,幽门螺杆菌全菌抗原和致病性大肠杆菌K-12为20.0ng/ml,CEA为12.5ng/ml,P53、Ras21和QYC抗原为10.0ng/ml,蛔虫卵、钩虫卵和白细胞抗原为50ng/ml,对临床粪便样本采用该芯片检测结果表明上述检测抗原在不同疾患中有不同改变。通过对血红蛋白、幽门螺杆菌全菌抗原与其他厂家生产的同类产品比较发现粪便蛋白芯片的质量与同类产品接近。血红蛋白符合率95％,幽门螺杆菌全菌抗原符合率92％。结论粪便蛋白质芯片技术是临床粪便样本实验室检测的新手段之一,具有广阔的市场前景。
Aim To prepare and apply a concise biochip for detection of various antigens in stool. Methods Based on the principle of antigen-antibody affinity, we developed a concise biochip for detection of various antigens in stool including hemoglobin, albumin, the whole bacterial antigen of H, pylori, K-12 E. coli, CEA, p53, Ras21, QYC. bellyworm egg, hookworm egg and white cell in pyroxylin membrane. Results The protein biochips were diverse on sensitivity of various antigens, that of hemoglobin, albumin was 50ng/ml. 1000ng/ml, H. pylori and K-12 antigens were 20.0ng/ml, CEA was 12.5ng/ml, P53, Ras21 and QYC were 10.0ng/ml, and genes of bellyworm egg, hookworm egg and white cell were 50.0ng/ml, respectively. Furthermore, the biochip was applied to detection of the clinical stool samples for assessment of clinical value, The results indicated the biochip had excellent work in difference clinical stool samples. We obtained the coincident rate of 95% and 92% in hemoglobin and H. pylori antigen. Conclusions Biochip technique for detection of stool proteins is a new method and should have broad future market.
出处
《现代消化及介入诊疗》
2003年第1期25-27,共3页
Modern Interventional Diagnosis and Treatment in Gastroenterology
