摘要
目的探讨槲皮素在体外逆转耐药细胞株HL 6 0 /ADR的多药耐药。方法以HL 6 0 /ADR细胞为研究对象 ,用RT PCR法检测耐药基因MRP1的表达及槲皮素对其表达的影响 ;采用共聚焦激光扫描显微镜观察槲皮素作用前后柔红霉素 (DNR)在耐药细胞HL 6 0 /ADR内亚细胞结构分布的改变。结果HL 6 0 /ADR中有MRP1的过量表达 ,且槲皮素能下调该基因的表达 ;槲皮素能恢复DNR在HL 6 0 /ADR细胞中的异常分布 ,回归其作用靶点 ,逆转耐药。结论DNR在耐药细胞中的异常分布与肿瘤细胞耐药基因形成有关 ,槲皮素在体外直接抑制MRP1功能 ,恢复DNR在细胞内的分布。
Objective To investigate the effect of multidrug-resistance reversed (MDR) by quercertn in HL-60/ADR cell line in vitro. Methods We measure MRP1 expression of HL-60/ADR cell line by RT-PCR and study the relationship between quercetin and MRP1 expression. Using confocal scanning laser microscopy(CSLM), we observe the changes of subcellular distribution of DNR in HL-60/ADR before and after treatment with quercetin. Results MRP1 was overexpression in HL-60/ADRcell line and quercetin exerted a dose- dependent down-regulation on the expression of MRP1 gene. Quercetin restored the abnormal distribution of DNR in HL-60/ADR, made DNR return to their targets and reversed multidrug-resistance. Conclusion Altered subcellular distribution of DNR in resistant cell line was related to MDR gene formation. Quercetin could inhibit MRP1 function and could restore the subcellular DNR distribution.
出处
《上海第二医科大学学报》
CSCD
2004年第B11期9-11,21,共4页
Acta Universitatis Medicinalis Secondae Shanghai
