摘要
本项研究工作于1991年芦荟(Aloe)腋芽的组织培养基础上,利用生长点的组织培养中所产生的多芽为进一步提高快速繁殖的目的1992年研究了生长点的培养。取幼嫩茎尖做外植体,按常规组织培养法消毒灭菌,然后接种在分化培养基上(MS1000ml+GA_3(?).1mg+ZEATiN0.25mg)诱导生长点的生长同时丛生苗,然后长出小叶片的无根苗移栽到生根培养基上(1/2MS1000ml+IBA1.0mg+蔗糖20g)诱导根系。然后经过炼苗移栽到苗床。苗床培养土比例为砂:厩肥:壤土=1:1:1成活率可达80%。
After culturing aloe's axillary bud in 1991,we started to culture alo- e's growth bud in order to increase reproductive rate in 1992。Taking young stem apex as explant,after sterilization by routine method we inoculated it in differential culture medium MS1000ml+GA_30.1mg+ZEATIN0.25mg) to induct growth bud's growing and rostte seedlings。Then they produced small leaves but no root。We translat them in culture med- ium of striking roots(1/2 MS1000ml+IBA1.0mg+cane sugar20g)to induct roots。We translated them in the seedling bed finally,in which nutritive soil compained with sand,solid manure and soil dy1:1:1,after hard- ening seedling。Survival ratio is 80%.
