摘要
目的探讨重组人亲肝素性促轴突生长因子(rh-HBNF)是否有助于周围神经损伤后的恢复。方法按神经挤压伤模型建立大鼠坐骨神经损伤模型,采用抽签法随机分为治疗组和对照组,每组30只雌性SD大鼠。术后第1、2、3天分别将10μl(0.1mg)rh-HBNF和0.9%氯化钠注射液注射到治疗组和对照组大鼠的损伤神经周边,并于术后1、2、3、4周每组分别处死6只,检测各项临床和病理指标。两组各留6只,每周检测1次坐骨神经功能指数(SFI),至第10周。结果治疗组第2、3周的SFI恢复程度明显大于对照组,差别有显著性意义(均P<0.05);感觉神经再生距离第2、3周治疗组明显大于对照组(P<0.05);组织学检查神经远端横截面积、近端横截面积、神经通过率和有髓神经纤维直径治疗组均大于对照组(P<0.05或0.01)。结论rh-HBNF对周围神经损伤的恢复有促进作用。
Objective To determine the neurotrophic effect of rh-HBNF on regeneration of injured peripheral nerve in rats. Methods A sciatic injury model was induced in SD rats. Sixty female SD rats with induced sciatic injury were randomly divided into HBNF-treated group and control group. In the following 3 days 10μl (0.1mg) rh-HBNF and 0.9% Nacl were injected (q.d) to the injured sciatic nerve of both groups respectively. At the 1st, 2nd, 3rd, 4th weeks, six rats from both groups were sacrificed respectively, the pathological exams were performed. The sciatic function indexes(SFI) of remaining 6 rats in each group were examined till week 10. Results The SFIs in HBNF-treated group were higher than those in control group (P<0.05) at the 2nd and 3rd weeks; the results of pinch test were also higher in HBNF-treated group than those in control group at the 2nd and 3rd weeks (P<0.05). Histological findings showed that the cross-sectional areas of distal nerve and the proximal nerve, the nerve pass rate and the myelinated axon diameter in HBNF-treated group were all greater than those in control group(P<0.05 or P<0.01). Conclusion rh-HBNF may promote the recovery of the injured peripheral neural system.
出处
《浙江医学》
CAS
2005年第1期23-26,32,共5页
Zhejiang Medical Journal
