摘要
为探讨细胞核与细胞质调控减数分裂的机制,对昆明白小鼠GV期卵母细胞进行了GV移植研究.将6～8周龄小鼠GV期卵母细胞分别与6～8周龄和3月龄小鼠GV期卵母细胞进行GV互换,所形成的3种GV-胞质体复合体的融合率(85.0%～89.8%)和3种重组卵母细胞的成熟率(93.9%～96.2%)并不因小鼠年龄的改变而有所变化.经人工活化后,3种重组卵母细胞形成原核期胚和2-细胞期胚的比率(分别为80.5%～85.4%和51 2%～55 4%)也不因不同年龄小鼠卵母细胞所带来的细胞质或细胞核的改变而受到影响.细胞遗传学分析表明成熟的重组卵母细胞表现出正常的核型.
To find out the cytoplasmic and nuclear mechanisms regulating meiosis, GV transfer is carried out in GV oocytes of KM mouse. The electrofusion rates(85.0%～89.8%) are equivalent among 3 types of GV-cytoplast complexes constructed by GV exchange between 6～8-week-old mice and 6～8-week-old or 3-month-old mice. The maturation rates(93.9%～96.2%) are not different among 3 types of reconstructed GV oocytes because of change of maternal age. After maturation of reconstructed GV oocytes, the percentages of pronuclear-stage (80.5%～85.4%) and 2-cell embryos (51.2%～55.4%) developed from 3 types of reconstructed oocytes by artificial activation are not influenced by cytoplasmic or nuclear changes resulting from oocytes of different age groups of mice. Cytogenetic analysis shows that the mature reconstructed oocytes have normal karyotype.
出处
《烟台大学学报（自然科学与工程版）》
CAS
2005年第1期34-40,共7页
Journal of Yantai University(Natural Science and Engineering Edition)
基金
国家重点基础研究发展规划项目(973计划)(G1999055902).
关键词
卵母细胞
生发泡
核移植
oocyte
germinal vesicle
nuclear transfer
