摘要
目的 研究增殖细胞核抗原 (PCNA)和细胞周期蛋白依赖性激酶 2 (CDK2 )在gax基因抑制血管平滑肌细胞 (VSMC)增殖中的作用。方法 以携带大鼠gax基因表达序列的重组腺病毒载体 (AdCMV gax)转染VSMC后 ,检测 gax、PCNA和CDK2的表达及3 H TdR掺入量的变化。结果 AdCMV gax转染后 ,VSMC中Gax蛋白的表达比转染前显著增高 ;AdCMV gax转染后VSMC的PCNA和CDK2表达较未转染组显著降低 ;AdCMV gax转染使VSMC的3 H TdR掺入量显著降低。结论 gax基因抑制VSMC增殖的机制与其抑制PCNA和CDK2的表达有关。
Objective To investigate the role of proliferating cell nuclear antigen (PCNA) and cyclin dependent kinase 2 (CDK2) in inhibition of vascular smooth muscle cells (VSMCs) proliferation by gax gene. Methods After being transfected by AdCMV-gax, the expression of gax, PCNA and CDK2 in VSMCs were assessed. The effect of gax overexpressions on VSMCs proliferation was observed by 3 H-thymidine incorporation. Results After AdCMV-gax was transfected to VSMCs, the level of Gax protein expression was significantly higher than that before transfection. PCNA and CDK2 expressions were decreased after VSMCs were transfacted with AdCMV-gax. The level of 3 H-thymidine incorporation was decreased significantly in with AdCMV-gax transfected VSMCs compared with that in non-transfected VSMCs. Conclusion The mechanism of inhibition of VSMCs proliferation by gax gene is related with depression of expression of PCNA and CDK2.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2005年第2期139-141,共3页
Medical Journal of Chinese People's Liberation Army
