摘要
目的 探讨结核性胸膜炎发病的免疫学机制。方法 结核性渗出性胸膜炎患者19例,在抗结核治疗前首次所取胸液分离的淋巴细胞为胸液组;健康献血员19名外周静脉血分离的淋巴细胞为正常组;正常组淋巴细胞加入卡介苗提取物(斯奇)孵育为实验组。采用ELISA法测定上述各组淋巴细胞培养 48 h后上清液中γ干扰素(IFN- γ)、白介素 4(IL -4)的水平。结果 结核性胸膜炎患者胸液淋巴细胞培养上清液中IFN -γ水平较正常组明显升高(P<0.01),而IL- 4水平则较正常组有所下降(P<0.05)。正常人外周血淋巴细胞经斯奇康孵育的培养上清中 IFN -γ、IL- 4 水平与胸液组基本一致(P>0.05)。结论结核性胸液淋巴细胞主要以分泌 IFN -γ为主的Th1细胞占多数,表现为 Th1优势;而分泌 IL- 4 为主的 Th2细胞则处于相对弱势。这种极化现象是由与斯奇康具有相似抗原特性的结核分支杆菌菌体抗原来介导的。
ObjectiveTo explore the immunological mechanism of tuberculous pleurisy.MethodsNormal peripheral blood lymphocytes were taken from 19 healthy blood donors (normal group) and lymphocytes in pleural effusion were taken from 19 patients with tuberculous pleurisy before anti-tuberculosis treatment (effusion group).The normal peripheral blood lymphocytes were supplemented with polysaccharide nucleic acid fraction of Bacillus Calmette Guerin (PSN-BCG) as test group.The levels of interferon-γ (IFN-γ) and interleukin-4 (IL-4) in supernate of the lymphocytes were determined by ELISA assay after culture for 48 h.ResultsThe level of IFN-γ in supernate of the lymphocyte culture from tuberculous pleural effusion were significantly higher than those from normal group (P<0.01),but the level of IL-4 were lower than those in the normal group (P< 0.05).This level of IFN-γ in the test group were approximately the same as those of the effuscion group (P> 0.05).ConclusionsThe lymphocytes in pleural effusion from patients with tuberculous pleurisy are mainly T helper lymphocytes-1 (Th 1) which secreted IFN-γ while Th 2 lymphocytes which secreted IL-4 are rare.This polarization phenomenon is mediated by tuberculous bacillus somatic (O) antigen.
出处
《中国抗感染化疗杂志》
2005年第1期42-43,共2页
Chinese Journal of Infection and Chemotherapy
