摘要
将穿梭载体 pRL1和 pRL10经双亲接合从大肠杆菌转入鱼腥藻中后,发现两种被修饰的穿梭载体 pRL1M 和 pRL10M。经限制酶图谱和 DNA 序列分析表明,pRL1M 和 pRL10M 中分别含有插入序列因子 IS2和 IS10。DNA 序列分析表明 IS10为 IS10-R 和 IS10-L 的杂交体。由于插入序列因子,使宿主对氯霉素的抗性增加。Southern 杂交表明在鱼腥藻的基因组 DNA中不含 IS10。
The pRL1 and pRL10 were transfered by the biparental conjugation from E.coli toAnabaena sp.strain ATCC27893 and two modified vectors,pRL1M and pRL10M,werefound.The insertion sequerce elements IS2 and IS10 were identified in pRL1M andpRL10M respectively by restriction maps and DNA sequenceing.The sequence of theIS10 from pRL10M appears to be a hybrid of IS10-R and IS10-L found at either end ofthe transposon Tn10.The resistance to chloramphenical of hosts was enhanced after theinsertion of IS2 and IS10.It was shown that there was no honologous between IS10 andthe genomic DNA of Anabaena by Southern hybridization.
出处
《微生物学报》
CAS
CSCD
北大核心
1993年第5期347-353,共7页
Acta Microbiologica Sinica
关键词
穿梭载体
鱼腥藻
大肠杆菌
Shutter vector
Anabaena sp.strain ATCC27893
