摘要
目的 探讨改进的小鼠腹部异位心脏移植技术的应用效果。方法 同系BALB c小鼠 80只 ,均为雄性 ,体重 2 3～ 2 7g。苯巴比妥纳 (70mg kg)行腹腔内注射麻醉。心脏移植 :在受体腹主动脉及下腔静脉游离后 ,用一弧形小动脉钳将上述血管一并钳夹阻断血流。在 2 5倍显微镜下用 11- 0的尼龙线将供心主动脉与受体腹主动脉 ,肺动脉与下腔静脉作端侧连续吻合。经阴茎背静脉注入林格氏液 0 3ml,然后松开阻断钳恢复血流。结果 成功施行心脏移植术 4 0例。受体手术时间为 (6 1 8± 6 5 )min ,血管阻断时间为 (2 8 6± 3 4 )min ,供体心脏缺血(36 2± 3 7)min。 7d成功率为 92 % (37 4 0 ) ,30d为 90 % (36 4 0 ) ,10 0d为 80 % (32 4 0 )。结论 改进的小鼠腹部异位心脏移植技术是一种简便、成功率高的方法。
Objective To observe the effects of the improved technique for hetertopic heart transplantation. Methods Syngenic male Balb/c mice(n=80, weighing 23 to 27g) were randomly divided into donors and recipients. All animals were anesthetized by an intraperitoneal injection of Pentobarbital (70 mg/kg). Heart transplantation: The recipient's abdominal aorta and inferior vena cava were carefully isolated and a small artery clamp with curve was placed across these vessels for blocking blood flow. Both anastomosis of the donor's thorax aorta to recipient's abdomenal aorta and anastomosis of donor's pulmonary artery to recipient's inferior vena cava were performed by using running 11-0 nylon sutures with the aid of 25 magnification microscope. Recipient mice were given 0.3 warm saline by injection via the dorsal penile vein before vascular clamping was removed. Results 40 heart transplantation were successfully performed. The recipient procedures took (61.8± 6.5) min. Vascular occlusion time was (28.6±3.4) min, and the donor ischemic time (36.2±3.7) min. Transplant success rates were respectively 92%(37/40), 90% (36/40), 80%(32/40) at 7 days, 30 days, 100 days postoperatively. Conclusion Improved technique for heterotopic heart transplantation in mice is a simple, high successful rate procedure.
出处
《中国比较医学杂志》
CAS
2005年第1期43-44,共2页
Chinese Journal of Comparative Medicine
