摘要
用一对根据已克隆的水稻OsPR-4b基因序列和水稻基因组序列数据库相应序列设计的引物扩增到OsPR-4b基因上游2257bp的启动子序列.用PLACE软件分析顺式作用元件,发现OsPR-4b基因起始密码子上游1.5kb的区域内存在几个可能与PR蛋白基因表达调控相关顺式作用元件,如W盒、PAL-boxA、GT-1结合序列、Dof结合序列和MybSt1元件等.将OsPR-4b基因启动子区域及其5'部分缺失构件与gus报道基因相连,并克隆进pCAMBIA1391z载体中,用于分析启动子活性以及顺式作用元件在调控OsPR-4b基因表达中的功能.
A 2257 bp promoter sequence upstream of the rice OsPR-4b was amplified using two primers designed on the basis of the sequence of OsPR-4b and rice genome sequences database. Several cis-acting elements, including W-box, PAL-boxA, GT-1 elements and MybSt1 elements, were recognized with the aid of PLACE program. The promoter region sequence and its 5' deletion constructs were fused to the gus reportor gene in pCAMBIA1391z vector for analyzing the promoter activity of the cloned promoter sequence and the functions of the cis-acting elements in regulation of OsPR-4b expression.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2005年第1期22-26,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家自然科学基金项目(30170494).
