摘要
目的 :观察体外羟基喜树碱、平阳霉素单独和联合应用对人舌癌Tca 8113细胞的生长抑制及端粒酶活性的影响。方法 :采用MTT法、软琼脂克隆形成法、透射电镜观察法、流式细胞术 ,TRAP PCR ELISA法研究。结果 :羟基喜树碱、平阳霉素单用及合用对Tca 8113细胞均有很好的抑制作用 ,且以合用效果最强 ,羟基喜树碱、平阳霉素使Tca 8113细胞的克隆形成率降低 ,超微结构、细胞周期发生明显变化 ,抑制端粒酶活性且有一定的时间依赖性。结论 :羟基喜树碱、平阳霉素对人舌癌Tca 8113细胞在体外有很好的抑制作用且联合应用效果更佳。
Objective:To study the effects of hydroxycamptothecine(HC PT ) and pingyangmycin(PYM) alone or in combination(HCPT/PYM) on the proliferation and telomerase activity of human tongue carcinoma Tca 8113 cells.Methods :The growth inhibitary effects of HCPT or PYM on Tca 8113 cells were stu died by MTT assay and the IC 30 values of the two drugs were obtained. Then the cells were exposed to HCPT or PYM at IC 30 , or to their combination. T he clonogenecity, cell cycle distribution, morphological change and telomerase a ctivity were studied by clonogenic assay, flow cytometry,transmision electron mi croscopy and telomeric repeat amplification protocol (TRAP) respectively.Results:IC 30 (ng/ml) of HCPT and PYM was 109 and 416 respectivel y, when in combination(HCPT/PYM) the IC 30 of HCPT and PYM was 35 and 100 r espectively. The clonogenecity(%) of the control, HCPT,PYM and HCPT/PYM treated cells was 31.7,11.6,15.2 and 4.1 respectively. PYM decreased S and G 2 phase ce lls,increased G 1 phase cells.HCPT or HCPT/PYM decreased G 1 phase cells and i ncreased S and G 2 phase cells.Drug treatment resulted in cell organ degenerati on and decrese of telomerase activity.The A value at A 450 of telomerase in control,HCPT,PYM and HCPT/PYM treated cells was 1.89±0.03,0.82±0.02,0.77±0.0 2 and 0.53±0.02 respectively(P<0.05).Conclution:HCPT and PY M have cytotoxicity effect to Tca 8113 cells and have stronger effect when used in combination.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2005年第1期78-80,共3页
Journal of Practical Stomatology
