摘要
目的从分子水平证实长时间航天搭载的番茄种子后代的遗传物质发生变异。方法利用俄罗斯“和平”号空间站搭载6年的番茄种子地面种植,进行随机扩增多态性DNA(randomamplifiedpoly morphicDNA,RAPD)检测。结果空间搭载的番茄和地面对照经RAPD分析,40个随机引物中,31个引物扩增的DNA带型一致,9个引物扩增的DNA带型表现多态性。共扩增出269条带,多态性带29条,多态性百分率为10.8%。空间搭载的番茄和对照相比扩增带型均出现差异,且空间搭载的番茄之 间的带型也不同。和地面对照相比,空间搭载的番茄中5号植株的DNA变异程度最大,3号植株变异程度最小。结论长时间航天飞行可引起番茄遗传物质DNA水平上的变异。
Objective To identify the variation of hereditary substance of tomato offspring from seeds under long-duration spaceflight condition. Method The tomato seeds carried in Russian MIR space station for six years, and the ground-based control were planted on the ground. The leaves of plants were used to do random amplified polymorphic DNA(RAPD) analysis. Result Forty random primers were used in this study, among which 31 primers generated the same DNA band type, and 9 primers generated different DNA band types. Forty primers amplified 269 DNA bands, among which 29 DNA bands were polymorphic ones with a percentage of polymorphism of 10.8%. Compared with the control, plants from seeds carried in space station generated different band types. Band types were different among five plants from seeds carried in space station. The number of polymorphic bands generated by plant No.5 compared with the control was the most, and that by plant No.2 was the least. Conclusion Long-duration spaceflight can cause variation of hereditary substance DNA of tomato.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2005年第1期72-74,共3页
Space Medicine & Medical Engineering
基金
国家863项目基金(2992AA744063)
关键词
空间诱变
番茄
随机扩增多态性
DNA分析
space mutation
tomatoes
random amplified polymorphy
DNA analysis
