摘要
目的建立变形菌随机扩增DNA多态性分型技术,应用于临床菌株流行病学调查。方法优化随机扩增DNA多态性指纹技术(RAPD)的实验条件,利用RAPD技术成功地检测21株变形菌DNA指纹图谱。结果21株变形菌分为18型别,其中16株奇异变形菌分为13个型,5株普通变形菌分为5个型。结论RAPD分型技术可简便、快速为变形菌提供分型标志,是分子流行病学研究的有效方法。
OBJECTIVE To establish the random amplification of polymorphic DNA (RAPD) typing technology for Proteus strains and apply it in the epidemiology investigation. METHODS To optimize the experiment condition of random amplification DNA polymorphic fingerprinting technique. Fingerprinting map of 21 Proteus strains were tested by RAPD. RESULTS Twenty one Proteus strains were divided into 18 distinct genotypes, among them 16 P. mirabilis strains were divided into 13 distinct genotypes and 5 from P. vulgaris strains. CONCLUSIONS RAPD provides markers for typing of clinical strains and it is suitable for molecular epidemiology studies with high typeability, simplicity and rapidness.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2005年第1期26-28,共3页
Chinese Journal of Nosocomiology
