摘要
目的筛选并分析胃癌发生、发展不同阶段的重要相关基因。方法应用抑制性消减杂交筛选胃癌差异表达基因片段,斑点杂交检测相关基因在胃癌发生、发展不同阶段的表达,半定量逆转录-聚合酶链反应方法验证检测结果。结果应用抑制性消减杂交筛选出26个胃癌差异表达基因片段,其中24个为已知基因,1个为新的表达序列标签,1个为推测基因。斑点杂交显示异型增生组织中AnnexinA2,RPS29,RPS12等基因表达增高;早期胃癌中RPS12等基因表达增高;在进展期胃癌及淋巴结转移癌中,细胞色素c氧化酶、铁蛋白轻链及RPS12等基因表达一致明显增高,胃癌组织中proteasome26S亚单位基因表达增高。其中RPS12基因在胃癌不同阶段表达一致增高。逆转录-聚合酶链反应实RPS12基因在胃癌中表达显著增高。结论发现了胃癌发生、发展过程中的一些重要相关基因,并初步证实RPS12基因可能在其中发挥更重要的作用。
Objective To screen and analyze the important associated genes in different stages of gastric cancer. Methods Using suppression subtractive hybridization (SSH) to screen differentially expressed genes; detecting the expression of genes in different stages of gastric cancer with dot blot hybridization; and verifying the results with semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR). Results Twenty-six differentially expressed gene fragments were obtained by means of SSH. Among them,24 were known genes,1 was a new expressed sequence tags (EST), and 1 was a hypothetical gene. The results of dot blot hybridization demonstrated that the expressions of Annexin A2, RPS29,RPS12 etc. in dysplasia were higher than those in normal mucosa; the expressions of RPS12 etc. in early cancer were higher than those in normal mucosa;the expressions of cytochromosome C oxidase II,ferritin light chain, RPS12 etc. in advanced gastric cancer and lymph node metastases were consistently higher than those in normal mucosa. The expression of proteasome 26S subunit gene in advanced gastric cancer was higher than that in normal mucosa. The expression of RPS12 was consistently higher in different stages of gastric cancer. It was demonstrated by RT-PCR that the expression of RPS12 in gastric cancer was higher than that in normal mucosa. Conclusion The authors have identified some important genes that might be involved in the carcinogenesis and progression of gastric cancer, and RPS12 may play more important roles in gastric cancer.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2005年第1期31-34,共4页
Chinese Journal of Medical Genetics
基金
国家重点基础研究发展规划项目(973)(G1998051203)
辽宁省自然基金(20032075)~~
