摘要
目的:探讨nm23-H_1和H-ras基因对大肠癌淋巴结转移的预测价值。方法:采用原位分子杂交法检测57例大肠癌组织中nm23-H_1、H-ras的表达状况。结果:nm23-H_1的阳性表达率为42.1％(24/57),H-ras阳性表达率为61.4％(35/57),与组织学分类无相关性;Dukes A+B组的nm23-H_1表达率显著高于C+D组(P<0.05),而H-ras则相反。结论:nm23H_1和H-ras基因的表达率与大肠癌淋巴结转移率分别呈正负相关(P<0.005),可用于淋巴结转移状况的预测,nm23-H_1的灵敏度为82.8％(24/29),H-ras的灵敏度为79.3％(23/29),联合检测则可相对提高预测的灵敏度(87.5％)。
Purpose: To study the relationship between the expression of nm23-H_1 gene and H-ras gene and the clinico-pathological characters in the colorectal cance. Methods: In 57 cases, in site hybridization analysis of nm23-H1 cDNA Probe. Results: The rate of positive expression of nm23-H1 was 42. l%(24/57), and 61. 4%(35/57) for H-ras without association between the degree of histology and expression of the genes- The percentage of positive expression for nm23-H1 in Dukes'A and B was higher than that in Dukes'C and D (P<0. 05), but for the expression of H-ras there was a inverse association (P< 0. 05). Conclusion: In the prediction of lymph node involvement, the semsitivity of nm23-H1 was 82. 8% and that of H-ras was 79. 3%. The sensitivity of prediction may be risen in combination with these results of two genes (87. 5%).
出处
《临床肿瘤学杂志》
CAS
1997年第4期24-26,共3页
Chinese Clinical Oncology
