摘要
目的 探讨被转入的人SR AI基因的整合与复制情况及其对小鼠繁育的影响。方法 采用类似系祖建系的方法 ,对人SR AI转基因小鼠的 2、35 34、35 6 0、36 38、36 39五个系列进行了繁育。并用PCR和Southernblot的方法 ,检测五个系列小鼠尾组织的DNA样品。结果 五个系列小鼠共产仔 431只 ,PCR检出阳性小鼠 178只 ,阳性率为 41.2 %。在 36 39系的F1、F2、F3代及纯合子转基因鼠中PCR阳性率分别为 47.8%、71.3%、75 %和 10 0 %。结论 人SR AI基因在子代鼠中能稳定遗传 ,且对生殖和发育无明显影响。
Objective To investigate human SR AI transgenic integration and reproduction and its effect on mice breeding. Methods Human SR AI transgenic mice models 2, 3534,3560,3638,3639 have been bred by means of creating the system similar to the system ancestry. PCR and Southern blot techniques were used to test the DNA samples of tail tissues in the 5 lines of mice. Results In 431 baby mice of the 5 lines, 178 were tested by PCR to be positive (41.2%) while the positive rates of generations F 1, F 2, F 3 in line 3639 and pure zygote transgnic mice were 47.8%,71.3%,75% and 100% respectively. Conclusion Human SR AI gene can steadily heritable in the mice sub generation and doesn't influence obviously their reproduction and growth.
出处
《中国实验动物学报》
CAS
CSCD
2001年第3期129-133,共5页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金资助项目 (编号 :3 990 0 0 61)
