摘要
探讨五羟色胺 (5 HT)含量在丹参改善单侧颞叶缺血性损害大鼠空间记忆障碍中的变化。方法 采用立体定向光化学技术选择性地导致大鼠左侧颞叶皮层缺血 ,术前 30min及术后第 3天分别给丹参组大鼠腹腔注射丹参 10g kg体重 ,用Morris水迷宫及图像自动监视系统监测大鼠行为。然后取脑组织进行病理观察及 5 HT免疫组化分析。结果 经丹参治疗 ,颞叶缺血性损害大鼠的空间记忆障碍得到显著改善 ,颞叶缺血性损害显著减轻 ,缺血灶内 5 HT表达明显减少。结论 在丹参改善单侧颞叶缺血大鼠空间记忆障碍中 ,5 HT起一定作用。
Objective To study the effects of radix saiviae miltiorhizae(RSM) on spatial memory of rats with ischemia of left temporal lobe and the expression of 5 HT.Methods Selective ischemia of left temporal lobe of rats was induced by the photochemistry method and RSM group was treated with RSM 10 g/kg iv 30 minutes before and 3 days after the operation.Morris water maze and Morris Maze Experimental Assistant System were used to monitor the behavior.Then the rat brains were observed pathologically by HE stain and immunonistochemisty of 5 HT.Results RSM significantly improved the spatial memory of rats with ischemia of left temporal lobe,reduced the ischemic damage and down regulated the expression of 5 HT in ischemic focus. Conclusion RSM could improve spatial memery ability of rats with ischemia of temporal lobe.The down regulation of the expression of 5 HT probably is related to the neuroprotective mechanism.
出处
《中华老年心脑血管病杂志》
CAS
2000年第2期120-123,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
军队医药卫生"九五"课题资助项目! (96M12 5 )
关键词
色胺类
丹参
脑缺血
记忆障碍
tryptamines
saivia miltorrhiza
cerebral ischemia
memory disorders
