摘要
CFL基因是从黄瓜中克隆到的拟南芥LEAFY(LFY)同源基因。以离体黄瓜子叶培养物成花为实验体系,利用mRNA原位杂交技术对CFL基因在花芽和营养芽分化过程中的时空表达进行了分析。结果如下:在花芽分化过程中,CFL基因在花原基形成、花器官原基分化及各轮花器官形成之初强表达,在花器官形成以后表达减弱或不表迦在营养芽分化过程中,CFL基因在分生组织、叶原基和幼叶中有明显表达,在成熟组织中不表达。结果说明CFL基因的表达在黄瓜子叶节花芽和营养芽分化中原基的分化形成是必需的。结果提示CFL基因可能参与细胞分裂调控和启动、营养性分生组织向花分生组织转变等过程。
CFL gene, a LFY homologue, was cloned from cucumber (Cucumis sativus L.). In this paper; in situ hybridization was performed to analyze the expression pattern of CFL gene at the stage of floral and vegetative buds differentiation in cucumber cotyledonary nodes cultured in vitro. The results showed that at the stage of floral differentiation, CFL gene was strongly expressed in primordia, floral organ primordia, and each whirl of floral organs at the early stage of their formation, but weakly expressed or not expressed in floral organs after their formation (Fig.2). At the stage of vegetative bud differentiation, CFL gene was strongly expressed in meristem, leaf primordium and young leaves, and no apparent expression signal was detected in mature tissues (Fig.3). The results suggest thatthe expression of CFL gene be necessary for the differentiation and formation of floral and vegetative primordias, and it plays an important role in floral and vegetative development in cucumber. The results also indicate that CFL gene involving in mitosis initiation, mitosis controlling, and transformation of vegetative meristem to floral meristem.
出处
《植物生理与分子生物学学报》
CAS
CSCD
2004年第6期644-650,共7页
Journal Of Plant Physiology and Molecular Biology
基金
浙江省自然科学基金(No.300030)杭州师范学院科研启动基金(No.101)资助。~~
关键词
黄瓜
子叶节
花发育
CFL基因
基因表达
cucumber
cotyledonary nodes
floral development
CFL gene
gene expression
