摘要
利用Affymetrix寡核苷酸基因表达谱芯片对 2型糖尿病肾病模型动物———db/db小鼠的肾脏基因表达谱进行了研究.在此基础上,利用末端快速扩增法和RT PCR的方法,对筛选出来的一个糖尿病肾病相关EST进行了cDNA克隆和表达分析.得到了一长为 1 7kb的cDNA片段.序列分析和网上数据库比对发现,此cDNA片段是小鼠表达序列AL0 2 30 0 1的一部分.根据AL0 2 30 0 1序列设计特异性引物,利用半定量RT PCR的方法对AL0 2 30 0 1在db/db小鼠肾脏、肝脏、脂肪、骨胳肌、脑等组织中的表达情况进行了分析,发现AL0 2 30 0 1在db/db小鼠肾脏中的表达情况与基因芯片检测结果吻合,且AL0 2 30 0 1在肝脏、脂肪、骨胳肌和脑等糖尿病肾病相关联组织中均有差异表达.这些结果提示:AL0 2 30 0 1与db/db小鼠的糖尿病肾病具有相关性.上述工作有利于揭示AL0 2 30 0 1基因的功能。
Kidney gene expression profile of db/db diabetic nephropathy mice was analyzed by using Affymetrix oligonucleotide genechip. Mice diabetic nephropathy related genes (and ESTs) were found out. A 1.7 kb cDNA was amplified and cloned by using RACE method, based on the sequence of EST. Homologous analysis of the sequence was carried out. And the result shows that it is part of mouse expression sequence AL023001, which function is unknown. To further investigate the relationship between AL023001 and diabetic nephropathy, the function of AL023001, expression levels of AL023001 were analyzed quantitatively using RT-PCR method. Result of RT-PCR shows that AL023001 is expressed differentially in liver, muscle, brain and fat, while the expression pattern of AL023001 in kidney is Just like that revealed by gene chip. These results suggest that AL023001 is related to mice diabetic nephropathy, though its function and possible role played in the development of mice diabetic nephropathy still to be investigated.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2004年第9期834-840,共7页
Progress In Biochemistry and Biophysics
基金
军队 十五"重点课题资助项目(0 1Z0 13)~~
