摘要
在基体辅助激光解吸电离飞行时间质谱(Matrix＿assistedlaserdesorption/ionizationtime＿of＿flightmassspectrometry,MALDI＿TOFMS)分析中,使用合适的基体或基体添加剂,可以改善样品结晶的形状、颗粒大小和分布的均匀性、提高样品分子解吸电离的效率、增强质谱信号、延长样品出峰时间、增加样品出峰的重复性、提高分辨率、扩大质量测定范围,从而提高分析的灵敏度、谱图质量以及分析的速度和效率。研究结果表明,与MALDI＿TOFMS分析葡聚糖的常用一元基体2,5＿二羟基苯甲酸(2,5＿dihydroxybenzoicacid,DHB)相比,在2,5＿二羟基苯甲酸中加入3＿氨基＿4＿羟基苯甲酸(3＿amino＿4＿hydroxybenzoicacid,AHB)组成的DHB+AHB二元新基体测定相对分子质量为10000、未经进一步分级的高聚合度葡聚糖样品,不仅能使样品形成均匀的结晶、易于解吸电离;而且还可使检测的质量范围比用DHB+HIQ二元基体有所扩大,达到21000以上。
Using a novel binary matrix — 2,5_dihydroxybenzoic acid/3_amino_4_hydroxybenzoic acid (DHB+AHB), a dextran sample with a high polymerization degree (n>100) without being further fractionated was directly determined by the matrix_assisted laser desorption_ionization time_of_flight mass spectrometry,and the molecular weight of the dextran up to more than 21 000 has been detected. The novel binay matrix was also compared with eight binary matrixes which have been reported before. The results showed that the larger mass range could be obtained and the dextran molecules were desorbed and ionized more efficiently and the reproducibility of spectra was enhanced when a novel binary matrix—DHB+AHB was used, also the resolution of spectra was increased.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第1期57-60,共4页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
广东省自然科学基金资助项目(990209)
