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凝胶色谱法研究配体与人血清蛋白质竞争铍的作用

Study on the Competition of Ligans with Human Serum Proteins for Be(Ⅱ) by Gel Chromatography
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摘要 用凝胶色谱法和原子吸收分光光度法.研究了有毒金属铍与人血清蛋白质的结合情况,及20种配体与蛋白质竞争铍的作用情况。结果发现:①在生理 pH 条件下人血清蛋白质能与铍强烈地结合,平衡时被蛋白质结合的铍量达95%,②有几种配体能使结合在蛋白质大分子上的铍下降到78%~18%,成为镀的小分子配体络合物。并且通过结构上分子基团的变换与竞争能力的比较,发现了有竞争能力的配体分子在结构上的规律性。 The binding of Be to human serum proteins and the competition of 20 ligands with proteins for Be were studied by gel chromatography and atomic absorption spec- trophotometry.The results showed that Be (?) was bound by serum protein strongly un- der physiological pH condition and the binding rate was about 95% at equilibuium condition. Among these ligand studed,several ligands could compete effectively with serum protein for Be and about 78%~18% of Be may thus be removed from proteins and form complex of small molecular ligand of Be.The structural regularity for the carboxylic acid ligands was found through changing substitution groups and compairing their competi- tive capacity.
出处 《首都医学院学报》 1993年第2期106-111,共6页
关键词 凝胶色谱法 铍中毒 解毒药 toxic metal beryllium Be gel chromatography complexing agents for removing metals human serum proteins
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