摘要
芦荟色酮是在芦荟叶中特有的一类具有抗炎和抑制酪氨酸酶等作用的活性物质。以芦荟全叶为原料,经过一系列的预处理手段,从脱色剂活性炭中获得芦荟色酮粗提物,再经过溶剂分配和富集后采用高速逆流色谱(HSC CC)对其中的色酮成分进行分离纯化。研究结果表明,采用氯仿 甲醇 水(体积比为4∶3∶2)混合溶液和二氯甲烷 甲醇 水(体积比为5∶4∶2)混合溶液作为溶剂分离系统,经过两步HSCCC可以分离纯化出色谱纯度在95%以上的芦荟色酮单体。经过紫外检测、快原子轰击质谱及核磁共振等方法的结构分析鉴定,证实分离所得物质为肉桂酰基 C 葡萄糖甙芦荟色酮。
Aloe chromone is a group of anti-inflammatory and anti-tyrosinase constituents found in aloe vera leaves. High-speed countercurrent chromatography (HSCCC) is reported for the preparative isolation and purification of a chromone from aloe vera. The crude extract was obtained by a series of pretreatment of aloe vera leaves and extracted from decolorizing active carbon with methanol. Then the extract was distributed between dichloromethane and water, and the organic part was then subjected to HSCCC for the isolation of chromone constituents. The chromone compounds with a high performance liquid chromatographic grade (>95%) was isolated through two step HSCCC separations by employing two solvent systems composed of chloroform-methanol-water and dichloromethane-methanol-water at volume ratios of 4/3/2 and 5/4/2, respectively. The chromone was finally identified as cinnamoyl-C-glycoside chromone by ultraviolet (UV), fast atom bombardment mass spectrometry (FAB-MS), nuclear magnetic resonance (()~1H NMR and ()^(13)C NMR).
出处
《色谱》
CAS
CSCD
北大核心
2005年第1期96-99,共4页
Chinese Journal of Chromatography
基金
教育部科学技术研究重点项目
北京市教委科技发展计划项目(KM200310011045).
关键词
高速逆流色谱
芦荟色酮
分离纯化
high-speed countercurrent chromatography
aloe chromone
isolation and purification
