摘要
目的:观察家兔再造阴茎植入神经轴浆流恢复情况,以探讨感觉神经末梢再生以及阴茎再造后感觉功能恢复的可能性.方法:将游离隐神经移植体与阴茎背神经吻接后植入腹壁浅血管筋膜蒂岛状皮瓣内,以建立阴茎再造重建感觉功能的动物模型.术后不同时间切取S2～S4节段双侧背根神经节(dorsal root ganglia,DRG),应用辣根过氧化酶法(horseradish peroxidase method,HRP)对实验组(植入神经)和对照组(未植神经)进行逆行神经示踪,观察DRG内辣根过氧化酶法(HRP)阳性标记细胞出现的时间和数量.结果:术后1个月实验组动物吻接侧DRG内开始出现HRP阳性细胞,且随着时间延长,阳性细胞数逐渐增多,植入隐神经束也可见HRP阳性;而对照组术后始终未见DRG内HRP阳性标记细胞出现.术后1个月、3个月和6个月3个时间点的HRP阳性神经元计数结果:S2为35.0±9.3、97.0±12.5、106.6±17.9,S3为22.1±7.7、71.3±16.7、103.8±29.4,S4为17.8±6.5、35.0±7.1、81.1±6.2.结论:家兔阴茎再造术后,植入神经轴浆流恢复,提示其感觉神经末梢再生与感觉功能重建是可能的.
Objective:To observe the axoplasm flow restoration in regenerative nerve fiber and to investigate the regeneration of sensory nerve endings and the restoration of sensory function in the reconstructed penis in rabbits.Methods:Animal models were established as follows:one segment of dissociative saphenous nerve was end-to-end anastomosed to pudendal nerve and then embedded in a superficial epigastric faciovascular pedicle flap to reconstruct penis.HRP retrograde tracing method was performed postoperatively to observe the restoration time and quantity of HRP positive neuron in dorsal root ganglion (DRG) in the nerve implantation group and the control group. Results: HRP-positive neurons in DRG appeared 1 month after operation and the number of the labelled neurons increased gradually wiuh the prolonged survival time in experimental group,while the HRP-positive cell was not scanned in the control group all the time after operation.The numbers of HRP positive neuron in the 1 month group,3 months group and 6 months group were S 2(35.0±9.3,97.0±12.5,106.6±17.9),S 3(22.1±7.7, 71.3± 16.7, 103.8±29.4) and S 4(17.8±6.5,35.0±7.1,81.1±6.2) respectively.Conclusion:The implanted nerves in the rabbit reconstructed penis have resumed axoplasm flow.It is suggested that both sensory nerve ending regeneration and sensory function restoration are possible.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第1期21-23,共3页
Academic Journal of Second Military Medical University
基金
上海市科技发展基金(024119057)
关键词
阴茎再造
神经移植
神经再生
轴浆流
phallic reconstruction
nerve transplantation
nerve regeneration
axoplasm flow
