摘要
精子必须经过获能和顶体反应(AR)才具受精能力。利用获能和AR 前后精子细胞内和精子表面大分子的变化,可探索新的避孕办法和男性不育诊断及治疗的新途径。本文将正常人精子于体外在BWW-BSA 培养基中获能,用钙离子载体A 23187诱导人精子进行AR(以三重染色和金霉素荧光染色两种方法检测这些精子的AR 率为50%左右),然后用这些新鲜的人精子作为免疫原,制备了23个抗人精子单克隆抗体,其中21个为IgM,2个分别为IgG_1和IgG_2。根据23个单克隆抗体与经获能和AR 处理的精子以及未处理精子的不同免疫反应,将它们分为A(AR 精子反应组)、B(未处理精子反应组)和C(双重精子反应组)三组,并测定了这些单克隆抗体与人的某些白血病细胞系的交叉反应。
Sperm released from the male genitaltract must undergo capacitation and acroso-me reaction(AR)before binding to oocytemembrane.Changes of sperm componentshave been found after the capacitation andAR.Based on these changes,the new con-traceptive methods and the treatment of themale infertility might be approached.Inthe present study,in vitro capacitated hu-man sperm were induced to undergo AR inBWW-BSA medium with calcium ionop-hore A 23187.The fresh human AR spermin about 50% of sperm population assayedby triple stain technique and/or chlortetra-cycline fluorescence staining were used forimmunization.Twenty three murine hybri-domas secreting monoclonal antibodies(Mc-Abs)to non-treated(NT)and/or AR hu-man sperm have been obtained.Of them,21 McAbs were classified as IgM,othersas IgG_1 and IgG_(2a).Based on the immuno-reactions of 23 McAbs with NT and ARsperm,they were divided into three groups:group A reacted mainly with the AR spe-rm,group B with NT sperm,and group Cwith both AR and NT sperm.The crossreaction of these McAbs with human leu-kemia cell lines was detected by ELISA andthe discrepant reactions were observed.
出处
《实验生物学报》
CSCD
1993年第1期73-78,共6页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金
世界卫生组织
洛氏基金
关键词
精子
单克隆抗体
抗原
顶体反应
Human sperm.Capacitation.Acrosome reaction.Monoclonal antibody.Sperm antigen
