摘要
目的从益生菌功能性评价出发,探讨昂立植物乳杆菌(LPOnlly)的耐酸、耐胆盐性能及其相关的表型和基因型信息。结果LPOnlly在耐酸、耐胆盐性能方面,优于其他实验室保存菌株,在pH3、37℃培养3h后,LPOnlly活菌存活率达95%以上。在1.0%胆盐的MRS培养液中生长24h后,LPOnlly活菌存活率达95%以上。经表型及分子鉴定,LPOnlly为植物乳杆菌,其16SrDNA序列保存于GenBank,登录号为AY590777,与实验室保存的其他植物乳杆菌的基因DNA指纹图谱相似率达71%。
Objective The acid and bile tolerance properties of L.plantarum LP-Onlly and its phenotype and genotype were studied based on probiotic functional assessment.The result was showed that the acid and bile tolerance properties of LP-Onlly was superior to other strains stored in the our lab,and LP-Onlly showed more than 95% survival rate at pH 3 cultured 3 hours at 37 ℃ or in the presence of 1.0% bile salt cultured 24 hours.LP-Oplly had been identified to be L.plantarum with the analysis of phenotype and genotype,and its sequence of 16S rDNA was stored at GenBank,Accession No.AY590777.
出处
《中国微生态学杂志》
CAS
CSCD
2004年第6期345-347,共3页
Chinese Journal of Microecology
