摘要
建立角膜上皮细胞培养的方法，并观察电离辐射照射后的形态计量改变。用角膜组织块培养原代细胞并传至3代，以10、20、30和50Gy ^(60)Coγ射线对离体细胞样品进行照射，观察照后不同时间其形态学改变，并用图像分析仪对细胞核、细胞浆的面积及核浆比进行定量分析。发现正常的角膜上皮细胞呈多角形或棱形，胞核位于细胞正中或偏中；照射后细胞胞核、胞浆明显增大，胞质内可见空泡，界限不清，核空泡化，核膜破裂，核浓缩、碎裂等。形态计量结果证明照后细胞核与细胞浆明显增大(p＜0.01），其核浆比随照后时间的延长而减小(p＜0.01)，均以20～30Gy组最为明显，表明照射引起胞浆的改变(肿胀)比核更明显。本研究结果为进一步研究角膜上皮细胞的照射效应提供了基础。
The culture method of corneal epithelial cells in rabbits was developed. The morphological changes of the cells were observed after they were irradiated by 60Coγrays of 10Gy, 20Gy and 30Gy, and a quantitative analysis was made for the area of nucleus and cytoplasm as well as the ratio of nucleus and cytoplasm by using image analyzer. The nucleus of normal corneal epithelial cells was polygon or shuttle-like, and was located near the center of the cell. The nucleus and cytoplasm were remarkably enlarged (p<0.01) after irradiation, especially in the 20~30Gy group. The volume of cytoplasm was more remarkably enlarged than that of the nucleus, which changed with some deterioration effects. When the time prolonged after irradiation, the ratio of nucleus and cytoplasm of the cells was gradually decreased. The results may provide a basis for the further study on irradiation effect of corneal epithelial cells.
出处
《中国体视学与图像分析》
2001年第3期141-144,共4页
Chinese Journal of Stereology and Image Analysis
关键词
兔角膜上皮细胞
体外培养
照射
图像分析
：Corneal epithelium
Rabbit
Cell culture
In vitro
Irradiation
Image analysis.
