摘要
采用间接免疫荧光法(IF)、碱性磷酸酶抗碱性磷酸酶桥联酶标法(APAAP)及病毒分离同时对78份下呼吸道感染患儿的鼻咽分泌物标本进行 RSV抗原检测。结果三者的检出率分别为41%、46%和31%。IF 与 APAAP 总检出率之间无显著差别,二者的敏感度与特异度相近,敏感度分别为88%和92%,特异度分别为93%和91%。后者因避免了 IF 需要较昂贵的荧光显微镜及标本不易保存缺点而适于在我国基层医院使用,值得推广。
78 nasop- haryngeal (NP) aspirates were obtained by suction from hospitalized children with pneumonia or bronchiolitis.Immunofluorescence (IF) and alkaline phosphatase antialkaline phosphatase (APAAP) for detection of respiratory syncytial virus(RSV) in NP aspirates were compared wi- th viral culture,the sensitivity and specificity of the two rapid methods in relation to culture were similar——88% and 93% for IF,92% for AP- AAP.
关键词
呼吸道
合胞病毒
免疫技术
Immunofluorescence
AlKaline Phosphatase AntiAlkaline PhosPhatase
Syncytiotoxin
