摘要
以自发性高血压大鼠 (SHR)为模型 ,Wistar Kyoto大鼠 (WKY)为正常对照 ,探讨过氧化物酶体增殖物激活受体γ (PPAR γ)表达水平 ,在高血压肥厚心肌中的变化及其意义 .4周龄 (4w)、 16周龄 (16w)SHR及WKY称量体重 (BM )后取心脏 ,分别测量左心室 (LV)、室间隔 (IS)、右心室 (RV)湿重 (WM ) ,并应用免疫组织化学技术 ,检测大鼠心肌细胞PPAR γ表达 ;应用蛋白质印迹和RT PCR技术 ,检测 4w、 16wSHRLV、IS、RVPPAR γ蛋白质和mRNA水平 .发现 4wSHRLVWM /BM、ISWM /BM、RVWM /BM与同龄WKY相比无显著性差异 (P >0 0 5 ) ,PPAR γ的蛋白质和mRNA表达水平亦相似 (P >0 0 5 ) ;16wSHRLVWM/BM和ISWM/BM较同龄WKY明显增高 (P <0 0 1) ,而PPAR γ的蛋白质和mRNA水平显著降低 (P <0 0 1) ,16wSHRRVWM/BM与 16wWKY相比差异无显著性 (P >0 0 5 ) ,PPAR γ的蛋白质表达水平亦无显著性差异(P >0 0 5 ) ,mRNA水平较WKY略减弱 (P <0 0 5 ) .实验结果提示 ,长期压力负荷过重导致SHRLV和IS心肌代偿性肥厚 ,肥厚心肌中PPAR γ蛋白质和mRNA表达水平显著降低 ,推测心肌细胞PPAR γ表达受抑制 ,可能参与了高血压心室重塑的发生机制 .
In order to investigate the varied expression of peroxisome proliferator-activated receptor-γ(PPAR-γ) in hypertrophied ventricle induced by pressure overload, the abundance of PPAR-γ in ventricles of spontaneously hypertensive rats (SHR) were detected. Using age matched Wistar-Kyoto rats (WKY) as control, the body mass (BM), left ventricle (LV) wet mass (WM), interventricular septum (IS) WM and right ventricle (RV) WM from 4-week-old and 16-week-old SHR were detected respectively. Then the protein expression in ventricles of WKY and SHR was detected by immunohistochemistral techniques and Western blot, and the mRNA level was analyzed by reverse transcription-polymerase chain reaction (RT-PCR). The ratio of LVWM, ISWM and RVWM to BM in 4-week-old SHR were similar to that in 4-week-old WKY (P>0.05), and the protein and mRNA level of PPAR-γ in ventricles of 4-week-old SHR were also similar to that of age matched WKY (P>0.05). In 16-week-old SHR, the ratio of LVWM and ISWM to BM were higher compared with age matched WKY (P<0.01), whereas there was no significant difference in RV (P>05). The protein and mRNA level in LV and IS of 16-week-old SHR were great lower than that of 16-week-old WKY (P<0.01), while in RV there was no significant difference of protein (P>0.05), and a little decrease of mRNA (P<0.05). The findings suggested that the expression level of PPAR-γ in hypertrophied ventricles induced by pressure overload (LV and IS in 16-week-old SHR) was significantly decreased, and the decreased expression of PPAR-γ in ventricles might contribute to ventricular remodeling in hypertension.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2004年第10期941-946,共6页
Progress In Biochemistry and Biophysics
关键词
PPAR-Γ
高血压
心肌肥厚
自发性高血压大鼠
peroxisome proliferator-activited recepter γ(PPAR-γ), hypertension, ventricular hypertrophy, spontaneously hypertensive rats