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瘦素对成骨细胞骨保护蛋白mRNA表达的影响初探 被引量：2

Regualtion of osteoprotegerin mRNA levels by leptin in human osteoblast-like cells
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摘要 目的初步探讨瘦素(Leptin)对成骨细胞骨保护蛋白(OPG)mRNA表达的影响。方法用SYBR Green Ⅰ染料实时监测定量RT-PCR的方法测定骨保护蛋白(OPG)mRNA表达。结果 瘦素(Leptin)使成骨细胞骨保护蛋白OPG mRNA表达呈剂量和时间依赖性降低。结论 瘦素(Leptin)作为一种内分泌因子,可直接与成骨细胞上leptin受体特异结合,通过受体后信号转导下调节成骨细胞OPG mRNA表达,使OPG mRNA表达降低,和其它因子共同参与骨再建调节。 Objective To study the effect of leptin on the expessione of osteoprotegerin (OPG) mRNA in osteoblast-like cells. Methods The expression of OPG mRNA in osteoblast-like cells was determined by real-time quantitative RT-PCR based on Light Cycler technology. Results Leptin down-regulated the mRNA levels of OPG (P<0.05) in a dose-and time-dependent manner. Conclusions Leptin acts directly on osteoblasts to regulate bone remodelling by decreasing OPG mRNA.
作者 李晓佳 魏松全 李双庆 安振梅 杨元
机构地区 四川省人民医院神经内科 四川大学华西医院内分泌科 四川大学华西医院遗传研究室
出处 《中国骨质疏松杂志》 CAS CSCD 2004年第4期429-431,419,共4页 Chinese Journal of Osteoporosis
关键词 MRNA表达 OPG 骨保护蛋白 成骨细胞 瘦素 LEPTIN受体 影响 调节 定量RT-PCR 内分泌 <Keyword>Leptin Osteoblasts Osteoprotegerin
分类号 R681 [医药卫生—骨科学] R336 [医药卫生—外科学]
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参考文献19

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同被引文献22

  • 1齐菲,颜光涛,林季,薛辉,王录焕.类风湿关节炎急性期患者血清中瘦素及相关因子水平的变化[J].中华风湿病学杂志,2006,10(6):353-355. 被引量：8
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  • 7Madiehe AM, Mitchell TD, Harris RBS, et al. Hyperleptinemia and reduced TNF-secretion cause resistance of db/db mice to endotoxin[J]. J Clin Invest,2005,115 : 1659 -1665. 被引量：1
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中国骨质疏松杂志
2004年 第4期

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