摘要
本文报道应用人结肠癌建株LoVo细胞接种裸鼠,并鼠间移植传代,建立人结肠癌(HCC)裸鼠移植瘤模型。接种和传代的移植瘤具有相对稳定性,其成活率达100％。通过对移植瘤大体形态观察和病理组织学、兔疫组化、透射电镜检查,以及^(131)I标记抗CEA单抗的体内放免定位显像和免疫毒素导向抗肿瘤一系列实验研究表明这一模型无论从形态结构、病理特征、生物学标志和免疫学功能都与原发性肿瘤极为相似,因此对于肿瘤细胞胚胎基因再表达机制和肿瘤抗原的分析,以及肿瘤的实验诊断和导向治疗研究应用具有重要意义。
An experimental tumor model was established in nude mice with subcutaneous innoculation of LoVo cells, a human colonic tumor cell line. It was observed that the tumor xenograft could be stably passed down through generations with a hundred percent viability. By using techniques of immunohistochemistry and ultrastructure analysis, it was further revealed that the characteristics of the xenotransplanted tumor were similar to the original ones in terms of morphological and pathological features, biological markers, as well as immunological functions. In addition, we were able to show that the tumor in the nude mice could be localized exactly with 131I-labelled anti-CEA MAb. Based on this observation an immunotoxin developed by conjugating the CEA MAb with Trichosanthin, a cell-toxic plant protein purified from a Chinese medicinal herb, was tested for targeting treatmtnt of the tumor xenograft, which resulted in 70% suppression of the tumor growth.
出处
《上海免疫学杂志》
CSCD
北大核心
1993年第1期1-5,共5页
Shanghai Journal of Immunology
关键词
免疫毒素
导向治疗
结肠肿瘤
xenotransplanted human colonic tumor nude mice immunotoxin targeting treatment
