摘要
目的 建立稳定的新生大鼠耳蜗柯替器的培养方法 ,为内耳的研究提供新的条件和模型。方法 采用显微解剖技术完整地分离出新生大鼠整个耳蜗基底膜组织 ,分别采用组织贴壁法及立体定向法进行培养。结果耳蜗柯替器组织块培养 2 4小时后 ,组织外周即可见明显新生上皮及成纤维细胞 ,内、外毛细胞及支持细胞等结构清晰可见 ;经丫啶橙和碘化丙啶双重荧光染色进行细胞活性鉴定 ,毛细胞及组织活性良好。结论 用组织贴壁法及立体定向法进行耳蜗柯替器体外培养是一种理想的耳科实验造模方法。
Objective To establish reliable tissue culture methods of organ of Corti in neonatal Wistar Rat.Methods The organ of corti was aquired from Wistar Rat with the method of microdissection.The tissues were cultured by two different methods.Results Twenty-four hours after the tissue cultivation,new epithelia cells and fibroblast were found around the tissue.Inner hair cell、outer hair cell and supporting cell grew well.The tissue was stained by Acridine orange and Prodium iodine.It became green,meaning the tissue had good activity.Conclusion The culture of organ of corti we introduced is an a ideal method for otology research.
出处
《听力学及言语疾病杂志》
CAS
CSCD
2005年第1期53-54,66,i001,共4页
Journal of Audiology and Speech Pathology
关键词
大鼠
柯替器
组织培养
Rat
Organ of corti
Tissue cultivation
