摘要
目的探讨缓释非洛地平(波依定)对感觉神经损伤性盐敏感性高血压大鼠的作用及其机制。方法感觉神经损伤性Wistar大鼠(3周龄)22只,分成3组给予含盐不同的饮食及波依定(30mg/kg·d,灌胃)干预4周,8只对照组大鼠给予高盐饮食4周,检测鼠尾收缩压、体重、淋巴细胞胞浆游离钙([Ca2+]i)、血浆降钙素基因相关肽(CGRP)和24h饮水量、尿量、尿钠、尿钾。结果波依定干预组比辣椒辣素+高盐组的鼠尾收缩压、淋巴细胞[Ca2+]i明显降低,肾排钠量明显增加。结论波依定干预组能阻止感觉神经损伤性处理大鼠在高盐饮食时鼠尾收缩压升高,淋巴细胞[Ca2+]i增高及肾排钠功能受损,提示该模型细胞内钙超载可能与细胞外钙内流有关。
Objective To investigate the effect and mechanism of felodipine extended release tablets (Plendil) on a novel salt-sensitive hypertensive model induced by sensory denervation. Methods Twenty two sensory denervated Wistar rats(3 weeks) were divided into 3 groups and subjected to the following treatment for 4 weeks: normal salt diet (0.5%, CAP-NS), high salt diet(4%,CAP-HS), high salt diet + Plendil (30 mg/kg·d, by orally,CAP-HS-PLE). Eight control rats were treated with high salt diet(CON-HS). Tail-cuff systolic blood pressure, body weight, intralymphocytic [Ca^2+ ]_i, plasma calcitonin gene-related peptide concentration (CGRP),24 hour water intake, urinary volume, urinary Na+ and K+ concentrations were examined. Results Tail-cuff systolic blood pressure and intralymphocytic [Ca^2+ ]i were lower in CAP-HS-PLE group than in CAP-HS group. SBP:(116.0±4.6 vs 143.4±6.6)mm Hg, [Ca^2+ ]i [(27.9±7.2 vs 133.7±15.1)nmol/L, P<0.001]. 24 hour urine sodium excretion was higher in CAP-HS-PLE group than in CAP-HS group [(8.75±0.69 vs 7.89±0.82)pg/mL, P=0.036). Conclusion Plendil can prevent the development of salt-sensitive hypertension, the overloading of intracellular [Ca^2+ ]_~i and the impairment of urinary sodium in sensory denervated Wistar fed a high-salt diet. This suggests that the overloading intracellular [Ca^2+ ]_~i in the model may due to the influx of extracellular calcium.
出处
《高血压杂志》
CSCD
北大核心
2005年第1期37-40,共4页
Chinese Journal of Hypertension
关键词
缓释非洛地平
盐敏感性
高血压
辣椒辣素
感觉神经
Felodipine extended release tablets(Plendil)
Salt-sensitivity
Hypertension
Capsaicin
Sensory nerves
