摘要
目的 探讨直肠癌中p2 7的表达和血清转化生长因子 β1(TGF β1)的水平 ,以及TGF β1对p2 7的调控关系。方法 对 37例直肠癌、2 2例直肠腺瘤和 19例正常对照者用免疫组化二步法检测其 p2 7的表达情况 ,用酶联免疫吸附法 (ELISA)测定血清TGF β1水平。结果 p2 7在正常直肠组织和直肠腺瘤组织中呈高表达 ,其表达阳性率分别为 89.4 7%和 90 .91% ,阳性表达定位于细胞核 ;直肠癌组织中 p2 7表达阳性率降为 6 4 .87% ,与前两者比较 ,差异有统计学意义 (P =0 .0 2 5 ) ,且伴有 p2 7细胞浆表达者占 4 5 .83%。血清TGF β1在正常对照组的表达阳性率为 2 1.0 5 % ,直肠腺瘤组为 2 7.2 7% ,而直肠癌组则升至 5 1.35 % (P =0 .0 4 5 )。p2 7的表达与直肠癌的分化程度、淋巴结转移和浸润深度有关 ;血清TGF β1表达水平与直肠癌的淋巴结转移、浸润深度和CEA水平有关。血清TGF β1阴性组中p2 7表达阳性率为 88.89% ,血清TGF β1阳性组中 p2 7表达阳性率降为 4 2 .11% ,差异有显著性意义 (Mantel Haenszelχ2 =6 .75 5 ,P =0 .0 0 9)。结论 p2 7对于判断直肠癌的恶性程度和预后有指导作用 ,血清TGF β1可作为直肠癌的辅助诊断指标 ,TGF β1可下调直肠癌中 p2 7的蛋白表达。
Objective To detect p27 expression in rectal carcinoma and serum transforming growth factor β 1 (TGF β 1) level in these patients, and to elucidate the modulatory effect of serum TGF β1 on p27 expression in rectal carcinoma. Methods Expression of p27 was measured in 37 cases of rectal carcinoma, 22 of rectal adenoma and 19 of normal control specimens by immunohistochemical staining using antibodies to p27. Serum level of TGF β1 was measured in these patients by enzyme linked immunosorbent assay (ELISA) method. Results p27 protein was expressed in normal rectal tissue, rectal adenoma and rectal carcinoma, and the positive rate was 89.47%, 90.91% and 64.87%, respectively. The positive rate of p27 in rectal carcinoma was significantly lower than that of normal rectal tissue and rectal adenoma ( P =0.025). p27 was mainly located in nucleolus of normal rectal tissue and rectal adenoma, and the positive rate of p27 in cytoplasm of rectal carcinoma was higher than that of normal and rectal adenoma. The positives rates of serum TGF β1 in normal group, rectal adenoma group and rectal carcinoma group were 21.05%, 27.27% and 51.35%( P =0.045),respectively. The expression of p27 related to histological differentiation, lymph node metastasis and infiltration depth. Serum level of TGF β1 related to lymph node metastasis, infiltrated depth and CEA level. The positive rate of p27 in TGF β1 negative group and positive group was 88.89% and 42.11%(Mantel Haenszel χ 2=6.755, P =0.009), respectively. Conclusion TGF β1 may be useful in assessment of malignance and prognosis of rectal carcinoma. TGF β1 can down regulate p27 expression in rectal carcinoma.
出处
《中国普外基础与临床杂志》
CAS
2005年第1期33-36,共4页
Chinese Journal of Bases and Clinics In General Surgery
