摘要
目的 探讨干扰素 (IFN)对人结肠癌细胞人类白细胞抗原DR(HLA DR)表达的影响。方法 采用免疫组化SP法和ELISA法分别检测不同剂量的α IFN和γ IFN刺激前、后HLA DR在 4株人结肠癌细胞HCT 8、Lovo、SW 4 80和Ls 174 T中的表达情况。结果 α IFN和γ IFN刺激之前 ,4株细胞中除Lovo细胞不表达HLA DR ,其他 3株细胞均可不同程度表达HLA DR ;刺激之后 ,4株细胞系的HLA DR表达均有所增强 ,并呈现出IFN依赖性及γ IFN的剂量依赖性 ,γ IFN的诱导作用强于α IFN。结论 IFN可以上调人结肠癌细胞HLA DR的表达 ,IFN用于结肠癌的临床治疗具有一定的应用前景。
Objective To inquire into the regulative effect of interferon (IFN) on human leucocyte antigen DR(HLA DR) expression in human colonic cancer cells. Methods The expression of HLA DR in 4 colonic cancer cell lines HCT 8,lovo,SW 480 and Ls 174 T was detected with immunohistochemical SP and ELISA method before and after being stimulated by different doses of α IFN or γ IFN. Results All cell lines except lovo cell were HLA DR positive before stimulation with α IFN or γ IFN. HLA DR expression was enhanced on all of the 4 cell lines after stimulation, and it was correlated with γ IFN and the dose of γ IFN. The effect of γ IFN was more obvious than that of α IFN. Conclusion IFN can enhance HLA DR expression in human colonci cancer cells and clinical therapy of IFN for colon cancer has a certain applying prospect.
出处
《中国普外基础与临床杂志》
CAS
2005年第1期15-18,共4页
Chinese Journal of Bases and Clinics In General Surgery
