摘要
目的 :研究CD4 4s及变异体v6与子宫内膜异位症 (EM)发病之间的关系。方法 :采用SP免疫组化方法检测CD4 4s及变异体v6在 2 0例卵巢异位子宫内膜及相应在位内膜和 2 0例正常内膜组织中的表达。结果 :①正常内膜腺上皮细胞在增生期不表达CD4 4s及变异体v6 ,分泌期大部分表达。②在位内膜腺上皮细胞表达规律与正常相似 ,但在增生期已有部分 (3/ 10 )出现CD4 4s表达。③卵巢异位内膜腺上皮细胞CD4 4s表达强度明显高于对照组 (P <0 .0 5 ) ,变异体v6表达强度与对照组比较差异无显著性。④间质细胞不管增生期还是分泌期只表达CD4 4s ,不表达变异体v6。卵巢异位内膜间质细胞表达强度明显强于对照组 (P <0 .0 5 )。结论 :①CD4 4s及变异体v6在正常子宫内膜腺上皮细胞中表达并呈周期性改变 ,推测其表达可能与卵巢激素调控有关。②异位内膜CD4 4s的表达增强可能与异位内膜黏附腹膜有关。
Objective:To study the relationship between the expression of CD44s and isoform v6 and the pathogensis of endometriosis.Methods:The expressions of CD44s and isoform v6 were detected with SP immunohistochemiscal technic in 20 cases of ovarian endometriotic tissues and matching eutopic endometriums and 20 cases of normal endometriums.Results:①The positive expressions of CD44s and isoform v6 were observed in most of secretory pattern of normal glandular epithelial cells and negative in a proliferative pattern.②The expressed regulation of CD44s and isoform v6 in glandular epithelium of eutopic endometrium was same as the normal group, but there was expression of CD44s in a proliferative pattern(3/10).③The expression intensity of staining of CD44s in glandular epithelium of ovarian ectopic endometrium was significantly higher than that in normal group (P<0.05).The expression of isoform v6 had no statistic significance compared with normal group.④The stroma was only positive for CD44s both in proliferative and secretory patterns, and negative for isoform v6. The expression intensity of staining of CD44s in stromal cells of ovarian ectopic endometrium was significantly higher than that in normal group(P<0.05).Conclusion:①The expression of CD44s and isoform v6 in glandular epithelium of normal endometrium shows a cyclic change, which means that its expression of CD44 may be correlated with ovary hormonal regulation.②The strong expression of CD44s in endometriotic tissue may play an important role in the adhesion between the endometriotic tissue and peritoneum.
出处
《温州医学院学报》
CAS
2004年第6期436-438,共3页
Journal of Wenzhou Medical College
