摘要
蛋白质在溶液中的三维空间结构、动力学与蛋白质生物功能的关系是在分子水平上理解生命现象的重要基础 .NMR技术在研究蛋白质动力学方面具有独特的优势 ,所能表征的运动过程相关时间尺度很广 .文章综述了异核多维NMR技术研究蛋白质动力学的实验技术和理论方法 ,介绍了描述蛋白质动力学的内运动参量的意义和Model Free方法 ,并举例说明1
One of the fundamental problems in understanding life science at the molecular level is the relationship among structure, dynamics, and function of proteins. NMR has a unique capacity to investigate dynamics properties of proteins over a range of different time scales with atomic resolution. This review focuses on the experimental and theoretical methods used in heteronuclear spin relaxation measurement for studying protein dynamics, and the parameters describing the protein internal motion as well as the Model-Free method. Finally, some application examples are given in which ^(15)N relaxation measurements were performed to study the backbone dynamics of protein and protein-ligand complex.
出处
《波谱学杂志》
CAS
CSCD
北大核心
2004年第4期385-396,共12页
Chinese Journal of Magnetic Resonance
基金
中国科学院"百人计划"研究基金
上海市重点基础研究基金 ( 0 3JC140 81)
厦门大学预研基金资助项目
