摘要
端粒结合因子(TRF1和TRF2)及抑癌基因ING1表达异常减少与多种颅外肿瘤的发生、发展密切相关。本研究是为了探讨胶质瘤细胞TRF1、TRF2和ING1基因表达变化的相互关系及其在胶质瘤发生、发展中的作用。方法:采用mRNA原位杂交和免疫组化染色等方法观察了70例不同级别的人胶质瘤组织标本。结果:本组70例胶质瘤INGl mRNA、P33^(ING1)蛋白、TRF1蛋白和TRF2蛋白的阳性表达率分别为94.3%、88.6%、100.0%和98.6%,I～Ⅱ级组、Ⅲ级组及Ⅳ级组间比较它们的阳性表达率均无显著性差异(P>0.05)。表达INGl mRNA、P33^(ING1)蛋白、TRF1蛋白和TRF2蛋白的四种阳性肿瘤细胞密度彼此间均呈显著性正相关(r=0.739～0.847,P<0.001),并均随肿瘤级别升高而相应减少,不同级别组间比较差异均有显著性(P<0.01)。结论:以上指标对评价胶质瘤的生物学行为均有重要参考价值,胶质瘤细胞中TRF1蛋白及TRF2蛋白表达异常减少可能是导致其ING1基因表达下调的重要因素,它们的表达异常减少可能在胶质瘤发生及恶性进展过程中起重要作用。
BACKGROUND & OBJECTIVES: Abnormal decreased expression of telomere repeat bind-ing factor-1(TRF1), telomere repeat binding factor-2(TRF2) and inhibitor of growth-1 (ING 1) have been re-late closely to the development and progression of many extracranial malignant tumors. This study was undertaken to investigate the relationship among expression variations of TRF1, TRF2 and ING1 gene in human glioma cells as well as effect of these variations on the development and progression of the gliomas. METHODS: Expressions of ING1 mRNA, P33^(ING1) protein, TRF1 and TRF2 proteins in 70 human glioma specimens with different malignant grades were analyzed using in situ hybridization and immunohistochemistry. RESULTS: The expression of ING1 mRNA, P33^(ING1) protein and TRF2 protein were detected in 66(94.3%), 62(88.6%) and 69(98.6%) of 70 gliomas respectively. All of these gliomas expressed TRF1 protein (100%). Their expression incidences did not show the significant difference among different malignant grade gliomas (P <0.05). The quantities of the four kinds of positive cells were correlated positive-ly with one another (r=0.739～0.847, P< 0.001), and all of them were significantly more in WHO grade I～II gliomas than that of in grade III gliomas and the fewest in grade IV gliomas (P<0.01). CONCLU- SION: Our results suggest that all of the above parameters have the important referential value in the evaluation of biological behavior of gliomas. In glioma cells, abnormal decreased expression of TRF1 and TRF2 proteins may be important factors which result in down-regulation of the ING1 gene expression. The abnormal decrease of their expressions possibly plays the important roles in the development and malignant progression of gliomas.
出处
《中国神经肿瘤杂志》
2004年第4期260-264,共5页
Chinese Journal of Neuro-Oncology
基金
国家自然科学基金(No.39870335)
