摘要
目的 :探讨结外弥漫大B细胞淋巴瘤 (DLBCL)中BCL 6基因 5′非编码区的突变情况及与临床的关系。方法 :石蜡标本中提取DNA ,扩增BCL 6基因 5′非编码区 +6 5～ +5 12bp ,测序。同时进行回顾性分析。结果 :结外DLBCL中BCL 6的突变率为 13 6 4 % (6 / 4 4 ) ,突变与年龄、性别、有无B症状、肿瘤大小、临床分期、血清LDH水平、BM浸润、是否CR无相关性 ;突变者的生存时间与未突变者相比无统计学差异 ,但有延长的趋势 (平均生存时间分别为 74和 6 5 0 2个月 ) ;突变不是影响预后的独立危险因素。结论 :BCL 6基因 5′非编码区点突变可能部分参与了结外弥漫大B细胞淋巴瘤的致病 ,突变者较未突变者相比有生存期延长的趋势。
Objective:To analyze the mutation of 5′ noncoding region of BCL 6 gene in extranodal diffuse large B cell lymphoma (DLBCL) and the relationship between mutation and clinical characteristics.Methods:Extract DNA from paraffin bedded extranodal DLBCL cases,then PCR and sequenced.Meanwhile retrospective analysis was done.Results:The mutation rate was 13 64%(6/44).The clinical characteristics,such as age、sex、B symptom、bulky tumor mass、stage、the level of LDH、BM involvement and CR,had no significant influence on BCL 6 mutation;the survive time of DLBCL with/without BCL 6 mutation had no obvious difference,but there was a tendency the survival time of mutated patients was longer than that of non mutated (the mean survival time was 74 and 65 02 months,respectively);mutation wasn't an independent prognostic factor.Conclusion:The mutation of 5′ noncoding region of BCL 6 gene maybe partly involves in the mechanism of extranodal DLBCL. [
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2005年第1期49-51,共3页
Chinese Journal of Immunology
