摘要
本文研究了3-氰基吡啶微生物转化产物的反相高效液相色谱(RP-HPLC)分析方法。采用径向加压C_(18)分离柱和甲醇-H_2O(20∶80)为流动相,在研究选定的流动相速度梯度色谱条件下,底物3-氰基吡啶和产物尼克酰胺、尼克酸得到了全分离,其最低检出量分别为1×10^(-9)、1.5×10^(-10)和1.0×10^(-10)g。此法分析周期短,重现性好,操作简便,可同时完成底物和产物的分离分析,适用于批量微生物样品的分析。
The conditions for the analysis of transformed products of 3-cyanopyridine via microorganism by RP-HPLC with flow rate gradient of mobile phase have been studied. Under the optimum conditions.the biotransformed products, nicotinamide and nicotinic acid. and 3-cyanopyridine as a substrate were well separated. When the AUFS was 0. 05, the minimum detectable amounts of 3-cyanopyridine, nicotinamide and nicotinic acid were 1× 10-9g,1. 5× 10-10g and 1×10-10g respectively.
出处
《色谱》
CAS
CSCD
北大核心
1993年第2期76-78,共3页
Chinese Journal of Chromatography
