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不同饲毒法测定Bt8010毒力效价研究

STUDIES ON BIOASSAY FOR TOXICITY OF BT 8010 USING TWO KINDS OF INFECTIVE FEED
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摘要 以棉铃虫初孵幼虫为供试虫,用合成饲料和天然饲料两种饲毒法,CS3ab-1991(毒力效价Ha15000IU/mg)为标准品对Bt8010粉剂进行毒力测定。饲毒后的供试虫在30℃恒温下培育72小时。合成饲料饲毒法测定的CS3ab-1991LC_50=108.6±10.8ug/ml,Bt8010LC_50=197.1±15.6ug/ml,毒力效价8273.1±715.8HaIU/mg.天然饲料饲毒法测定的CS3ab-1991LC_50=406.3±47.5ug/ml,Bt8010LC_50=705.0±86.8ug/ml,毒力效价8633.2±871.8HaIU/mg。 The toxicity of wettablc powder Bt 8010 was tested by using the newly hatching larvae of cottonbollworm as test insect and Bt CS3ab-1991(15000 Ha IU/mg)as the standard. The infective larvae wereincubated at 30 ℃ for 72h.The rcsults showed that LC_50 of Bt CS3ab-1991 was 108.6ug/ml(r=0.07)andLc50 of Bt 8O10 was l97.1± 15.6ug/ml(r=0.98),the toxicity of Bt 8010 was 8273,1±7l5.8 Ha IU/mg(C.V.=87%)by using the infective method of the synthetical feed.When tested by using the infectivc meth-od of the natural feed, LC_5O of Bt 8010 was 705.0± 86.8 ug/ml(r=0.97),and LC_50 of Bt CS3ab-1991 was406.3 ± 47.5 ug/ml(r=0.96).Therefore,the toxicity of Bt 8010 was 8633.2 ± 871.8 Ha IU/mg(C.V=10.2%).
出处 《华东昆虫学报》 1994年第2期85-88,共4页 Entomological Journal of East China
关键词 LC50 毒力测定 天然饲料 效价 棉铃虫 幼虫 培育 Bacillus thuringiensis 8010,bioassay,Heliothis armigera,infective feed.
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