摘要
目的;分析体外培养扩增及直接消化分离的松果体细胞微囊的功能差异,筛选最佳的种子细胞来源。方法:游离并摘取乳鼠松果体,胰酶、胶原酶消化分离获取松果体细胞悬液,将直接消化细胞与体外培养一定时间的松果体细胞同时进行海藻酸钠一多聚赖氨酸一海藻酸钠(APA)微囊化处理,采用形态学观察、细胞计数、高效液相色谱(HPLC)技术检测并分析两种细胞微囊的活性和功能差异。结果:体外培养后囊化的松果体细胞分散良好,细胞增殖旺盛,囊内细胞的褪黑素(MT)分泌功能逐渐恢复;直接消化包裹的细胞分散程度较差,有细胞聚集现象,增殖不明显,分泌功能呈下降趋势。结论:体外培养的松果体细胞与微囊材料组织相容性好,囊内细胞活性和功能均优于直接消化包裹的细胞微囊。
Objective: To explore the differences of biological characteristics between encapsulated pinealocytes uncultured in vitro in advance and those cultured before encapsulating. Methods: To remove the pineal glands of neonatal rats under operating microscope and isolate pinealocytes through collagenase and trypsin digestion. Pinealocytes cultured one week in vitro and collected immediately after digesting were encapsulated in APA microencapsulation and cultured in vitro respectively. Morphological appearances of two groups of capsules were observed, while cell counting and HPLC technique were used to analyze the proliferation and function of them. Results: Pinealocytes in capsule cultured in advance scattered well and showed higher ability of proliferation than those uncultured before capsulating. The level of melatonin secretion raised gradually for the former, however, the secretion ability of pinealocytes in capsule uncultured in advance in vitro was not productive. Conclusions: Comparing with cell capsule uncultured in advance in vitro, the surviving and functional characteristics of pinealocytes in APA microencapsulation cultured in advance are more competent.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2004年第6期641-644,共4页
Chinese Journal of Clinical Anatomy
基金
广东省科技计划攻关项目(2002C31306)
关键词
APA微囊
松果体
细胞培养
褪黑素
APA microencapsulation
pinealocytes
cell culture
melatonin
