摘要
目的构建含有血小板衍化生长因子B(PDGFB)基因的组织工程化皮肤,进行动物移植实验,研究PDGFB基因表达在真皮血管重建中的作用。方法构建PDGFB真核表达质粒,用脂质体LipofectAMINE介导转染成纤维细胞。分别构建3种不同类型的组织工程化皮肤角质形成细胞+脱细胞猪真皮基质(A组),角质形成细胞+脱细胞猪真皮基质+成纤维细胞(B组),角质形成细胞+脱细胞猪真皮基质+PDGFB基因转染的成纤维细胞(C组),分别移植于大鼠背部创面,观察术后2、4、6周真皮血管重建情况。结果术后2周C组真皮浅层内可见较多新生毛细血管长入,B组次之,A组毛细血管长入较少(P<005);术后4周各组真皮浅层内毛细血管数逐渐增加,但C组毛细血管数仍明显高于B、A两组(P<005);术后6周各组织工程化皮肤内毛细血管数差异无显著性意义。结论PDGFB基因在组织工程化皮肤移植后早期真皮血管重建中发挥了重要的作用,为移植后皮片成活提供了保障。
Objective\ To study the effect of PDGF on dermal blood vessel reconstruction by transplanted tissue engineering skin containing PDGF B gene to rats.Methods The recombined eukaryotic expression vector, pcDNA3 1 hPDGF B, was constructed and transfected into fibroblasts mediated by LipofectAMINE. Keratinocytes+acellular dermal matrix (group A), keratinocytes+acellular dermal matrix+fibroblasts (group B), keratinocytes+acellular dermal matrix+fibroblasts with PDGF gene(group C) were recombined respectively, then transplanted them to rat dorsum and evaluated the reconstruction of blood vessels in the dermis after 2, 4, 6 week postoperation.Results In 2～4 weeks after skin grafting the vescularization rate in group C was higher than that of group B and group A. The vescularization rates in all groups had no significant differences in six weeks( P >0.05). Conclusion PDGF B gene plays an important role in reconstruction of blood vessels in the dermis at early tissue engineering skin grafting, which ensures the take of grafted tissue engineering skin.\;
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2004年第6期447-450,共4页
Chinese Journal of Plastic Surgery
基金
铁道部科技基金资助项目(J2000Z083)
江苏省社会发展基金资助项目(BS99049)
江苏省卫生厅科技基金项目(H200159)
