摘要
目的研究持续被动运动(CPM)对全层关节软骨缺损修复的影响。方法将60个兔膝关节建立全层关节软骨缺损模型,随机分为3组,术后A组每天进行CPM8h;B组每天进行CPM2h,2组均连续进行CPM4周,然后笼内自由活动至12周。C组仅笼内自由活动。分别于术后4周和12周处死各组半数兔,并取材,行HE、番红O以及Ⅰ、Ⅱ胶原免疫组化染色,再进行评分。结果A组全层关节软骨缺损修复的效果较B、C两组明显。结论兔膝全层关节软骨缺损后给予CPM干预可显著促进组织的修复,同时可促进Ⅱ型胶原和蛋白多糖的恢复。
Objective To study the effect of continuous passive motion (CPM) on the healing of man made full thickness defects of articular cartilage in the knee of rabbit. Methods A diameter of 3.2mm full thickness defect of articular cartilage in the facies patellaris of femur were made in 60 knees of rabbits. Then the rabbits were randomly divided into groups A,B and C. The animals in groups A and B were treated with CPM for 8 hours and 2 hours daily, respectively, for 4 weeks, while those in group C kept in cage for free activity only. Half of the animals of every group were sacrificed at the end of the 4th and 12th weeks. The H.E. staining, safranin O staining and immunohistochemical analysis of collagens I and II were conducted, the gross appearance and histological change were observed and scored. Results There was significant difference with regard to the score of gross appearance and histological change among groups A, B and C. Conclusion The healing of full thickness defects of articular cartilage in rabbits treated by CPM is better than that in rabbits without CPM treatment. CPM can promote tissue repair and stimulate synthesis of collagenⅡand the glycosaminoglycan.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2004年第11期644-647,共4页
Chinese Journal of Physical Medicine and Rehabilitation
