摘要
目的 :探讨粒细胞集落刺激因子 (G CSF)和干细胞因子 (SCF)预处理对骨髓间充质干细胞 (MSCs)向心肌细胞分化的影响。方法 :MSCs均采用全骨髓法提取 ,通过换液传代逐渐纯化细胞 ,取传 4代的MSCs,在共培养前用DAPI标记 ,心肌细胞在培养的第 3d与MSCs共培养。对照组 :未施加任何干预的MSCs与心肌细胞共培养 ;实验组 :联合使用G CSF和SCF对大鼠在体动员 ,提取其MSCs与心肌细胞共培养。共培养前测定心肌细胞的活力 ,在共培养第 3、4、5d用免疫荧光标记肌钙蛋白T ,分析DAPI MSCs向心肌细胞分化的百分率。结果 :在共培养的第3、4、5d ,G CSF和SCF处理的DAPI MSCs的分化率分别为 (1.2 4± 0 .16 ) %、(3.4 6± 0 .2 1) %、(7.2 1± 0 .11) % ,均显著高于对照组的 0、(1.2 7± 0 .13) %、(1.32± 0 .2 5 ) %。结论 ::G CSF和SCF对MSCs具有促分化作用。
AIM: To investigate the effect of pretreatment of Granulocyte Colony-Stimulating Factor(G-CSF)and Stem Cell Factor(SCF) on the differentiation of mesenchymal stem cells(MSCs) into cardiomyogenic cells. METHODS: The MSCs were isolated primarily from bone marrow of adult rat, and purified by passage culture. The 4th passage of MSCs was labled by DAPI . Then cardiomyocytes that have been cultured for 3 days were cocultured with DAPI-MSCs . The control group: The MSCs which have no intervention were cocultured with cardiomyocytes. The experiment group: The MSCs which have been pretreated by SCF and G-CSF were cocultured with cardiomyocytes. The activity of cardiomyocytes was detected before coculture. Troponin T was examined by immunocytochemical method in the 3th、4th、5th day of coculture separately. Then we analyse the percentage of the differentiation of MSCs into cardiomyogenic cells. RESULTS: DAPI-MSCs pretreated by SCF and G-CSF can be induced to differentiate into cardiomyogenic cells with higher positive rate of differentiation[(1.24±0.16)%、(3.46±0.21)%、(7.21±0.11)% in the 3th、4th、5th day separately]than the control group[0、(1.27±0.13)%、(1.32±0.25)%] . CONCLUSION: G-CSF and SCF could induce MSCs to differentiate into more cardiomyogenic cells in simulative cardiomyocytes microenvironments in vitro.
出处
《心脏杂志》
CAS
2004年第6期501-504,共4页
Chinese Heart Journal
基金
湖北省教育厅重大项目 (2 0 0 3Z0 0 1)
关键词
骨髓间充质干细胞
心肌细胞
分化
mesenchymal stem cells
cardiomyocytes
differentiation
