摘要
为了更好地使IL-3在体内发挥其造血促进作用和免疫增强作用,于本实验中建立了IL-3基因疗法的小鼠模型;并动态观察了其外周血细胞数量的变化.通过基因转染、G418抗性筛选、有限稀释及IL-3活性测定,从16株NIH3T3成纤维细胞克隆中选出一株分泌IL-3高达2416U/ml的克隆株.将其移植入小鼠腹腔后,实验小鼠血清可检测出一定水平的IL-3并维持至半个月之久.实验小鼠外周血白细胞,特别是中性粒细胞数量显著增加,血小板也有不同程度的升高.该实验结果表明成纤维细胞途径能将IL-3基因携至体内进行有效表达并发挥显著的生物学作用.
To exert the hematopoietic and immunoregulatory effects of IL-3 more effectively, the murine model of fibroblast-mediated IL-3 gene therapy was established, and the changes of peripheral blood cells and splenocytes were observed. By gene transfection, G418 resistant screening, limiting dilution and IL-3 determination, a highly IL-3-secreting transfectant (2416U/ml, named as NIH3T3-IL-3+) was obtained from 16 positive clones. After NIH3T3-IL-3 were implanted i.p. into mice, certain levels of IL-3 could be detected in serum, which lasted over half a month. The peripheral WBCs, especially neutrophils, increased markedly. Besides, peripheral platelets also increased to various degrees. Our results showed that fibroblast-mediated gene therapy could transfer IL-3 gene into mice and express IL-3 effectively, which would exert significant biological activities.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
1995年第1期16-20,共5页
Chinese Journal of Cancer Biotherapy
基金
本文受国家自然科学基金优秀中青年人才专项基金(39421009)资助
