摘要
用单或双染色免疫荧光技术经流式细胞仪分析，研究一组重症肌无力(MG）患者外周血淋巴细胞表型的表达。结果表明志者组CD16，56阳性(CD16，56)细胞百分率显著低于健康对照组(分别为9．92±6．88和18．08±8．02，P<0．05)。未经治疗的患者，CD3^+，CD29^+ CD4^+细胞百分旱和CD4／CD8比值均高于对照(依次为69．48±10．43．和60．80±12．35，32．88±5．90和25．77±4．38，1．79±0.84和1.39±0.44．P值均<0．05)。CD8^+及CD16，56^+细胞百分率均低于对照组(分别为22．47±7．09和29．26±8.64．8．80±5．50和18．08±8．02．P<0．05和P<0.01。胸腺摘除患者，CD3^+和CD8^+细胞百分旱与对照组无显著性差异，CD16．56^+、CD29^+ CD4^+细胞百分率及CD4／CD8比值均低于对照组(依次为P<0．05，P<0．01和P<0.05)。随访7例患者胸腺摘除术前、后淋巴细胞表型的变化，进一步显示手术后CD3^+、CD4^+.CD29^+CD4^+细胞和CD4／CD8比值下降．而CD8^+细胞百分率升高.
Surface phenotypes of peripheral blood lymphocytes from patients with myasthenia gravis(MG)were investigated by flow cytometry in single or dual color staining-direct immunofluorescence.The results showed that there was a significant decrease in the percentage of CD16, 56￣+ cells(9.92±6.88%)in MG patients.In the patients without immune therapy,a significant increase in the percentages of CD3￣+(69.38±10.43%),CD29￣+ CD4￣+(32.88±5. 90%)cells and the ratio of CD4/CD8(1.79±0.84),but a significant decrease in percentages of CD8￣+ (22. 47±7.09)and CD16, 56￣+(8.80±5.50)were found when compared to healthy controls(CD16, 56+ 18.08±8. 02%,CD3￣+ 60. 80±12. 35%,CD29+CD4+ 25.77±4. 38%,CD4/CD8 1.39±0. 44, respectively).In the patients treated by thymectomy,the percentage of CD16, 56￣+,CD29+CD4+ and CD4/CD8 ratio were significantly decreased compared to controls.There were no significant defferences in the percentages CD3￣+ and CD8￣+cells between patients with thymectomy and controls.The data,from 7 cases followed before and after operation showed that there was a significant decrease in the percentages of CD3￣+, CD4￣+, CD29￣+CD4￣+ cells and CD4/CD8 ratio, but a significant increase in percentage of CD8+ cells after thymectomy.
出处
《中国神经免疫学和神经病学杂志》
CAS
1994年第2期79-84,共6页
Chinese Journal of Neuroimmunology and Neurology
基金
卫生部研究经费资助
