摘要
用０．０２ｍｌ／Ｌ的三羟甲基胺基甲烷（用磷酸调ｐＨ值至７）和甲醇（含２％乙酸和０．２５％庚烷磺酸钠）作流动相进行梯度洗脱，建立了脂性油膏药物中黄芩甙含量的反相高效液相色谱分析方法。本法分析速度快、重现性好，黄芩甙的平均回收率为１０３．７％。
A HPLC method for the analysis of baicalin in ointment is presented. Eluted by a gradient mode from 50% A (0.02 mol/L Tris, adjusted to pH=7 with phosphoric add) and 50% B (methanol, containing 2% gladal aCetic add and 0.25% sodium heptane-sulfonate) to 100% B, quanhtiative separation of baicalin with other components in the ointment is accomplished.
出处
《分析测试技术与仪器》
1994年第4期54-56,共3页
Analysis and Testing Technology and Instruments
