摘要
为了有效地从鱼病组织中初提纯草鱼出血病病毒，我们探讨了各种离心方法和离心参数，结果表明最佳离心方法为角度离心（日立２０ＰＲ－５２Ｄ离心机，ＲＰＲ２０－２转头），最佳离心参数为：转头温度是８℃，离心速度是１６０００转／分，离心时间是４．５小时。该离心方法和离心参数已成为我们实验室最常规使用的提纯该病毒的方法。
For more effichat isolation of Grass Carp Haemorrhage Virus(GCHV) from infected fish tissue,.differenth methods and different parameters of centrifugation were extlored. It was found that the most suitable centrifugation method angular centrifugation and the most suitable Parameters for isolating typical viral particles were: revolution speed 16000rpm; duration.4.5h, rotor temperatrre, 8℃ (Hitachi 20 PR-52D Centrifuge, RPR 20-2 Rotor). Thiscentrifugation method is now used routinely in our laboratory for the isolation of GCHV.
出处
《分析测试技术与仪器》
1994年第2期33-36,共4页
Analysis and Testing Technology and Instruments
